内皮素3在不同毛色绵羊皮肤中的差异表达

doi:10.11843/j.issn.0366-6964.2015.11.026

Abstract

Abstract:

To investigate the function of endothelin 3（Edn3） in sheep skins and the regulation of hair color formation，the differential expression of Edn3 in skins of black versus white sheep was examined.Quantitative real-time PCR（qRT-PCR），Western blotting and immunohistochemistry methods were performed.The results showed that the quantity expression of Edn3 mRNA in black sheep was 2.89 times than in white sheep（P<0.01）；the average protein expression of Edn3 in black sheep skin was higher than in white sheep skin on the optical density（P<0.01）.The results of immunohistochemistry revealed that Edn3 was localized in the hair dermal papilla，lower of hair bulb and outer root sheath in hair follicles of sheep with white hair color，but was localized in lower of hair bulb and outer root sheath in hair follicles of sheep with black hair color.The differential expression and localization of Edn3 in skin of white versus black sheep suggests a potential role in hair color regulation.

CLC Number:

S852.1

YANG Shan-shan，BAI Jun-ming，FAN Rui-wen，GENG Jian-jun，JIAO Ding-xing，ZHOU Ying，DONG Chang-sheng. The Different Expression of Endothelin 3 in Sheep Skins[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(11): 2111-2116.

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The Different Expression of Endothelin 3 in Sheep Skins

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